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KMID : 1140120080130030197
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Cancer Prevention Research
2008 Volume.13 No. 3 p.197 ~ p.204
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Protective Effects of Polygonatum odoratum Extracts on Amyloid β-protein-induced Death in Neuronal Cells
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Kim Eun-Sook
Choi Soo-Jin
Kang Ga-Hea
Oh Myung-Sok
Park Woo-Jin
Choi Young-Hwan
Lee Chul-Won
Lim Hak-Seob
Cho Yong-Kweon
Moon Ja-Young
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Abstract
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The amyloid β-protein (Aβ) is the principal component of the senile plaques characteristic of Alzheimer’s disease (AD) and elicits a toxic effect on neurons in vitro and in vivo. Many environmental factors including antioxidants and proteoglycans modify Aβ toxicity. In this study, we have investigated the protective effects of organic solvent-extracts of Polygonatum odoratum fractions pre-extracted with methanol on Aβ-induced oxidative cell death in cultured rat pheochromocytoma (PC12) cells. For this study, we used MTT reduction assay for detection of protective effects of organic solvent-extracts of Polygonatum odoratum fractions pre-extracted with methanol on Aβ25-35-induced cytotoxicity to PC12 cells. We also used cell-based β-secretase assay system to investigate the inhibitory effect of organic solvent-extracts of Polygonatum odoratum on β-secretase activity. We previously reported that methanol extracts of Polygonatum odoratum strongly attenuated cytotoxicity induced by the three A fragments (Aβ25-35, Aβ1-42 Aβ1-43) to both SK-N-MC and PC12 cells. In the present study, we found that the organic solvent extracts of Polygonatum odoratum fractions pre-extracted with methanol had the strong protective effects against Aβ25-35-induced cytotoxicity to PC12 cells and inhibitory potency to β-secretase activity. These results suggest that organic solvent extracts of Polygonatum odoratum fractions pre-extracted with methanol may contain the protective component(s) against Aβ-induced cell death in PC12 cells as well
as inhibitory component(s) to β-secretase activity. (Cancer Prev Res 13, 197-204, 2008)
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KEYWORD
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Amyloid β-protein, Polygonatum odoratum, Liliaceae, PC12 cell, BACE, β-secretase
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